Electron microscopy coating and cryogenic preparation

Cryo preparation techniques for cryo SEM and FIB/SEM are essential for the observation of biological, wet or beam sensitive specimens. Using cryo sem electron microscopy removes the need for conventional preparation methods, such as chemical fixation and critical point drying, and allows observation of specimens in their natural hydrated state.

Sputter coating is the standard method for preparing non-conducting or poorly conducting specimens prior to observation in a scanning electron microscope (SEM). Quorum offer low-cost, rotary-pumped sputter coaters for depositing non-oxidising metals – such as gold (Au) and platinum (Pt) – and turbomolecular-pumped coaters, suitable for both oxidising and non-oxidising metals, such as chromium (Cr).

The primary application of the glow discharge is to modify the surface of TEM grids in a way that it meets requirements for successful imaging of a variety of macromolecules. Integrated into one system, the two chambers enable the user maximum flexibility to choose which sample preparation technique they want to use: glow discharge in-air or in-chemical vapour, without downtime for cleaning or the risk of contamination and loss of samples.
 

The Quorum QuickLok provides an efficient and rapid way of transferring ambient temperature EM specimens – either under vacuum or in inert gas – into the vacuum chamber of SEM, FIB/SEM, beamline and other vacuum systems.
The Quorum K1050X RF plasma barrel reactor is designed for plasma etching, plasma ashing and plasma cleaning applications.
The E4800 Series of Recirculating Heaters/Chillers are recommended for a wide range of open and closed-loop temperature control applications.